Figure 3.

Primary transcripts detected by 5′tagRACE in IGR candidates. Names of oligonucleotides used to probe each RNA candidate are indicated at the top of each panel. Signs ‘+’ and ‘−’ refer to treated or untreated TAP RNA samples, respectively. Numbers on the right indicate lengths estimated for 5′tagRACE PCR products observed on 2.5% agarose gels. Amplicons below ∼80 bp were considered as aberrant products due to the average length of the specific primer (∼25–30 nt) and the 5′tag RNA adaptor (38 nt), (Supplementary Table S1). Asterisk: signals not sequenced or corresponding to an unrelated primary transcript as determined by sequencing. 5′tagRACE data obtained for rejected IGR candidates are shown in Supplementary Figure S2.