Table 3.
Catalytic activities of APAH mutants
| Mutant | Relative activitya | Relative activityb |
|---|---|---|
| wild-type | 1.00 | 1.000 |
| H158A | 0.03 ± 0.02 | 0.0107 ± 0.0008 |
| H159A | < 0.002c | < 0.0013c |
| Y323F | 0.01 ± 0.02 | 0.0018 ± 0.0008 |
a
Deacetylase activity measured using the standard polyamine deacetylase assay with acetylputrescine.
b
Deacetylasei activity measured using the Fluor-de-Lys deacetylase substrate used for HDAC assays, L-Lys(ε-acetyl)-coumarin.
c
Lower limit of detection. All measurements were made in triplicate.