Skip to main content
. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: J Immunol. 2011 Feb 25;186(7):3892–3898. doi: 10.4049/jimmunol.1003505

Figure 5.

Figure 5

Simultaneous blocking of the CaN/NFAT and Ras pathways blocks TCR-induced Gata-3 up-regulation. DP thymocyte from G3/MHC° mice or G3/MHC°/dnRas mice were stimulated overnight with 0.5 μg/ml αCD3/CD4 F(ab′)2 in the presence or absence of CsA (50 ng/ml), collected and analyzed by flow cytometry. The GFP expression levels were compared. Shown is the average and SEM of three experiments. A shows a representative histogram of the induction, and B shows the quantification of three independent experiments with a pair of G3/MHC° and G3/MHC°/dnRas mice per experiment.