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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Nat Immunol. 2010 Dec 5;12(1):29–36. doi: 10.1038/ni.1968

Figure 7. A RelA methyl-phospho switch at chromatin regulates NF-κB signaling.

Figure 7

(a) Requirement for PKCζ to decrease occupancy of RelAK310me1, GLP, and H3K9me2 at chromatin of RelA target gene promoters in response to NF-κB stimulation. ChIP assays of RelAK310me1, GLP, and H3K9me2 at promoters in Prkcz+/+ and Prkcz−/− MEFs23 ± TNF (20 ng/ml). y-axis: % input (see Fig. 2a). Negative control antibody ChIPs are shown in Supplementary Fig. 24. (b) Requirement for PKCζ in TNF-dependent induction of RelA target gene expression. Left: immunoblot analysis showing the absence of PKCζ protein in Prkcz−/− MEFs23. Right: amounts of Il1a and Il6 mRNA ± TNF (20 ng/ml) in the indicated cell lines were measured by RT PCR analysis. Error bars indicate ± s.e.m. from at least three experiments.