Fig. 2.
Inhibition of Notch signalling by N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) and Notch3 antibody decrease the collagen-specific T cell proliferation and attenuate T helper type 1 (Th1)- and Th17-type responses. Spleen mononuclear cells (SMNCs) from collagen II (CII)-immunized DBA/1J mice were restimulated with CII in the presence of DAPT (5 µM) or α-Notch3 (10 µg/ml). (a) The proliferation of SMNCs was determined by tritiated thymidine (3[H]-TdR) incorporation. Results were expressed as mean ± standard deviation of counts per minute (cpm) of triplicates or quadruplicates. (b) The percentage of Th1, regulatory T cells (Treg) and Th17 cells in SMNCs were analysed using flow cytometric intracellular staining.