Table 3.
Kinetic Properties of PTP1B against pY Peptides
| Entry No. | Peptide Sequencea | kcat (s−1) | KM (μM) | kcat/KM (μM−1 s−1) |
|---|---|---|---|---|
| 1 | ASSDDpYAA | 33 ± 1 | 18 ± 1 | 1.8 |
| 2 | DTADVpYAA | 31 ± 3 | 8.9 ± 1 | 3.5 |
| 3 | ARKRIpYAA | - | >500 | 0.14 |
| 4 | RRISTpYAA | 78 ± 2 | 90 ± 7 | 0.87 |
| 5 | DTADApYAA | 38 ± 2 | 35 ± 2 | 1.1 |
| 6 | DTAAVpYAA | 32 ± 1 | 18 ± 0.14 | 1.8 |
| 7 | DAADVpYAA | 32 ± 4 | 10 ± 1 | 3.1 |
| 8 | ATADVpYAA | 31 ± 1 | 12 ± 2 | 2.5 |
| 9 | AAAApYAAAA | - | - | 1.3b |
| 10 | AAAAApYEEVH | 34 ± 2 | 32 ± 7 | 1.1 |
| 11 | AAAAApYRHRR | 33 ± 3 | 234 ± 37 | 0.14 |
| 12 | RTLAApYAAAA | 33 ± 3 | 66 ± 17 | 0.51 |
| 13 | RTLAApYEEVH | 36 ± 0 | 43 ± 1 | 0.84 |
| 14 | RTLAApYRFQK | 37 ± 3 | 165 ± 18 | 0.22 |
| 15 | DTADVpYDWEF | 29 ± 5 | 5.2 ± 1 | 5.8 |
| 16 | DTADVpYGEFTI | 33 ± 3 | 7.0 ± 1 | 4.8 |
| 17 | AKFEDTpYAA | 17 ± 1 | 3.5 ± 1 | 4.9 |
| 18 | AVWEFpYpYAA | 26 ± 1 | 2.9 ± 0.6 | 8.9 |
| 19 | AYTEpYTpYAA | 17 ± 1 | 2.9 ± 0.5 | 6.0 |
| 20 | AWSpYADpYAA | 21 ± 1 | 0.91 ± 0.08 | 23.5 |
| 21 | AVpYADHpYAA | 24 ± 1 | 3.1 ± 0.6 | 7.8 |
| 22 | ApYFPIWpYAA | 20 ± 1 | 3.5 ± 0.4 | 5.9 |
| 23 | ApYYAESpYAA | 16 ± 1 | 3.1 ± 0.4 | 5.1 |
| 24 | ApYpYEVSpYAA | 19 ± 1 | 2.0 ± 0.4 | 9.5 |
| 25 | ApYpYWTApYAA | 26 ± 1 | 1.7 ± 0.2 | 15.1 |
a
All peptides contained an N-terminal acetyl group and a C-terminal amide.
b
Data from ref. 36. Data reported represented the mean ± SD from three or more independent sets of experiments.