. Author manuscript; available in PMC: 2012 Mar 29.

Published in final edited form as: Biochemistry. 2011 Feb 18;50(12):2339–2356. doi: 10.1021/bi1014453

Table 4.

Kinetic Properties of SHP-1 and SHP-2 against pY Peptides

Entry No.	Peptide Sequence^a	SHP-1			SHP-2

		k_cat (s⁻¹)	K_M (μM)	k_cat/K_M (μM⁻¹ s⁻¹)	k_cat (s⁻¹)	K_M (μM)	k_cat/K_M (μM⁻¹ s⁻¹)
1	WAGDDpYAA	57 ± 2	45 ± 5	1.3	36 ± 3	68 ± 15	0.53
2	FDIDIpYAA	33 ± 1	13 ± 1	2.5	30 ± 2	39 ± 5	0.77
3	EIFDFpYAA	32 ± 2	27 ± 4	1.2	26 ± 1	54 ± 6	0.48
4	FYDIDpYAA	66 ± 2	28 ± 2	2.4	50 ± 4	66 ± 13	0.75
5	SASASpYSASA	26 ± 4	1990 ± 420	0.013	42 ± 5	2300 ± 400	0.018
6	WDEDFpYSASA	37 ± 2	53 ± 10	0.70	50 ± 3	46 ± 10	1.1
7	SASASpYDWEF	12 ± 1	48 ± 9	0.25	35 ± 1	180 ± 7	0.19
8	WDEDFpYDWEF	75 ± 2	3.2 ± 0.3	23	43 ± 1	6.3 ± 0.4	6.8
9	WRKRFpYDWEF	1.7 ± 1.0	780 ± 640	0.0022	1.3 ± 0.2	560 ± 140	0.0023
10	WDEDFpYRWKF	42 ± 2	150 ± 15	0.28	73 ± 7	210 ± 35	0.34
11	WDEDFpYAA				48 ± 2	43 ± 2	1.1
12	FDEDFpYAA				30 ± 2	31 ± 6	1.0
13	ADEDFpYAA				28 ± 1	34 ± 2	0.82
14	DWEDFpYAA				30 ± 1	17 ± 2	1.7
15	DFEDFpYAA				37 ± 1	23 ± 2	1.6
16	DAEDFpYAA				30 ± 1	28 ± 3	1.1
17	AKFEDTpYAA	19 ± 2	58 ± 13	0.34
18	AVWEFpYpYAA	24 ± 2	7.5 ± 2	3.3
19	AYTEpYTpYAA	17 ± 1	9.1 ± 1	1.9
20	AWSpYADpYAA	13 ± 1	22 ± 5	0.58

All peptides contain an N-terminal acetyl group and a C-terminal amide. Data reported represented the mean ± SD from three or more independent sets of experiments.