. Author manuscript; available in PMC: 2012 Feb 1.

Published in final edited form as: J Am Soc Mass Spectrom. 2011 Jan 27;22(2):255–264. doi: 10.1007/s13361-010-0029-0

Table 2.

Summary table of losses around 82 Da from synthetic histidine-containing peptides. “Loss” refers to the measured mass difference between the charge-reduced precursor and the most intense isotopic peak

Peptide	Charge of precursor	Loss from +1 charge reduced	Loss from +1 charge reduced	Loss from +1 charge reduced in MS³^*
AGAHPLESHYR	2
	3	83.060	81.044	83.060
	4	84.067	82.054	84.067
ALANGFARSHALL	2
	3	83.060		83.04^a
AWLNEHR	2
	3	83.060		83.060
GFSQNTSLTQHQR	2
	3	83.060		83.060
GYYYLHR	2	82.055
	3	83.060		83.060
HAAAKAAAH	2	81.044
	3	83.060	81.046	83.060
HAAAKAAK	2
	3	83.060
HFLTLAPIK	2
	3	83.059		83.054
HTGTPLGDIPYGK	2
	3
LPISAAHpSpSKTR	2
	3	83.058

An ion trap was used to collect MS³ ETD scans of ALANGFARSHALL [M + 3H]^++•

MS³ scans were carried on doubly charged ETnoD products