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. Author manuscript; available in PMC: 2011 Apr 12.
Published in final edited form as: Science. 2005 Nov 24;310(5754):1642–1646. doi: 10.1126/science.1120781

Fig. 3.

Fig. 3

Gluconeogenic and lipogenic gene expression is elevated in LKB1-deficient livers. (A) The left panel shows qRT-PCR examining the expression levels of mRNA for indicated gluconeogenic genes from the livers of mice of indicated genotypes and conditions 3 weeks after Cre administration. Expression was normalized to hypoxanthine-guanine phosphoribosyl transferase (HPRT) and equilibrated to the lowest-value condition for each gene. The right panel shows immunoblot analysis of PGC-1α or eIF4E (loading control) from fasted mice of indicated genotypes 6 weeks after Cre injection. (B) qRT-PCR for lipogenic target genes on liver samples as in (A). Mice were either fasted or fed ad lib. The induction of lipogenic targets is lower under ad lib fed conditions than for mice re-fed after fasting conditions (49). qRT-PCR data represent the mean + SEM for samples analyzed in triplicate.