Booster immunization increases memory CS252-specific CD8 T cell numbers and rescues protection against P. berghei sporozoite challenge. A, Experimental design. B, Total number of CS252-specific CD8 T cells per spleen as detected ICS for IFN-γ. C, Total number of CS252-specific CD8 T cells per liver. B and C, Cumulative data (mean±S.E.M.) from 5–8 mice from three experiments. D, Percent of PBL that are CS252-specific CD8 T cells. Data (mean±S.E.M.) are from 20 mice per group from two experiments. B–D, Data was analyzed by unpaired two-tailed t-test. Mice were challenged with 1000 Pb sporozoites. Numbers to right of bar graph represents the percent of mice protected against the development of blood stage parasitemia (number protected/number challenged). Protection results were analyzed by Fisher’s exact test.