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. 2011 May 1;138(9):1863–1873. doi: 10.1242/dev.059287

Fig. 5.

Fig. 5.

AGO3 and PAPI associate with TRAL/ME31B complex in nuage. (A) PAPI immunoprecipitates from ovary lysates were probed with the indicated antibodies. Extract plus beads (−Ab) and antibody plus beads (−Extract) were used as negative controls for immunoprecipitation. (B) AGO3 immunoprecipitates from ovary lysates were probed with indicated antibodies. (C) TRAL colocalizes with PAPI and AGO3 in the nuage (arrowheads). (D) TRAL/ME31B and PAPI co-fractionated with AGO3 in the non-ribosomal fractions. Polysome fractionation of 0-12 hour embryo extract was performed in a 15%-50% (w/w) sucrose gradient. The fractions were probed with the antibodies indicated. (E) Embryonic extract was treated with EDTA prior to sucrose gradient fractionation. The fractions were probed with the antibodies indicated.