Skip to main content
. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Nat Med. 2010 Dec 12;17(1):96–104. doi: 10.1038/nm.2270

Fig.3. Validation of DSG2 as Ad receptor. “Gain-of-function” studies.

Fig.3

a) Transduction of human cell lines that express different DSG2 levels. Human erythromyeloblastoid leukemia K562 cells and Burkitt’s B-cell lymphoma BJAB and Ramos cells were infected with Ad3-GFP and Ad5/35-GFP at increasing MOIs and GFP expression was measured 18 hours later. N=3. Standard deviation was less than 10% for all data points. b) Ectopic DSG2 expression. Human histiocytic lymphoma U937 cells were infected with a lentivirus vector carrying the DSG2 cDNA under the control of the EF1α promoter. Stable DSG2 expression was detected in >98% of lentivirus transduced cells by flow cytometry. c) Attachment of 3H-Ad3 and 3H-Ad35 to Raji, U937 and DSG2-expressing U937 (U937-DSG2) cells. Note that Ad35 attachment is mediated through CD46 and can be blocked by soluble CD46 (data not shown). d) GFP expression after transduction of U937 and U937-DSG2 cells with Ad3-GFP and Ad5/35-GFP. n=3