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. 2011 Mar 24;12:158. doi: 10.1186/1471-2164-12-158

Table 3.

Threshold settings used for homolog mapping using Blastx/n

identical matches expect value
Blastx (mouse) ≥ 80% ≤ 1-20
Blastn (sheep) ≥ 90% ≤ 1-50
Blastn (cow) ≥ 97% ≤ 1-50

Only Blast matches fulfilling both conditions were considered as true orthologs.