Table 1.
Human and mouse HDL-miRNA signatures in health and atherosclerosis.
| Human HDL miRNAs | ||||||
|---|---|---|---|---|---|---|
| Normal | Familial Hypercholesterolemia | |||||
| Normalized Abundance | Top Ten | Top Ten | ||||
| miRNA | (+/−SD) | P/A | miRNA | (+/−SD) | P/A | |
| 1 | miR-135a* | 121.9+/−41.3 | 6 / 6 | miR-223 | 3014553.4+/−5939444 | 5 / 5 |
| 2 | miR-188-5p | 17.1+/−5.7 | 6 / 6 | miR-105 | 13671.6+/−26536 | 4 / 5 |
| 3 | miR-877 | 11.1+/−5.2 | 6 / 6 | miR-106a | 437.3+/−812.3 | 4 / 5 |
| 4 | miR-223 | 8.4+/−6.9 | 6 / 6 | miR-17 | 436.8+/−815.4 | 3 / 5 |
| 5 | miR-760 | 5.9+/−2.1 | 5 / 6 | miR-135a* | 223.7+/−347.6 | 5 / 5 |
| 6 | miR-375 | 5.4+/−1.6 | 6 / 6 | miR-372 | 170.3+/−203.3 | 2 / 5 |
| 7 | miR-106a | 5.1+/−3.1 | 4 / 6 | miR-489 | 170.3+/−204 | 2 / 5 |
| 8 | miR-138-1* | 4.7+/−1.1 | 6 / 6 | miR-24 | 123.8+/−192.4 | 3 / 5 |
| 9 | miR-625* | 4.7+/−3.2 | 3 / 6 | miR-193b | 120.3+/−194.4 | 2 / 5 |
| 10 | miR-17 | 3.5+/−1.1 | 4 / 6 | let-7g | 106.9+/−201.7 | 1 / 5 |
| Significant Differential Abundance on Familial Hypercholesterolemia HDL | ||||||
| miRNA | Increased Abundance Fold Change | p-value | miRNA | Decreased Abundance Fold Change | p-value | |
| 1 | miR-223 | 3780.64 | 0.020 | miR-625* | −10.95 | 0.005 |
| 2 | miR-24 | 65.27 | 0.002 | miR-520c-3p | −5.43 | 0.013 |
| 3 | miR-342-3p | 30.35 | 0.037 | miR-509-3p | −3.74 | 0.012 |
| 4 | let-7b | 12.27 | 0.043 | miR-573 | −3.58 | 0.007 |
| 5 | miR-412 | 9.51 | 0.006 | miR-632 | −3.30 | 0.004 |
| 6 | miR-222 | 8.20 | 0.016 | miR-188-5p | −2.71 | 0.005 |
| 7 | miR-106a | 7.85 | 0.045 | miR-572 | −2.48 | 0.008 |
| 8 | miR-218 | 7.80 | 0.010 | miR-323-3p | −2.47 | 0.012 |
| 9 | miR-191 | 4.66 | 0.009 | miR-135a* | −2.41 | 0.049 |
| 10 | miR-20a | 19.56 | 0.066 | miR-877 | −2.26 | 0.021 |
| 11 | miR-186 | 10.94 | 0.161 | miR-138-1* | −2.00 | 0.037 |
| 12 | miR-17 | 7.64 | 0.103 | miR-526b* | −5.85 | 0.051 |
| miRNA | OFF (+/−SD) | P/A | miRNA | ON (+/−SD) | P/A | |
| 1 | miR-639 | 1.83+/−1.6 | 2 / 6 | miR-105 | 13671.6+/−26536 | 4 / 5 |
| 2 | miR-584 | 1.76+/−0.9 | 3 / 6 | miR-489 | 170.3+/−204.0 | 4 / 5 |
| 3 | miR-643 | 1.2 | 1 / 6 | let-7g | 106.9+/−201.7 | 4 / 5 |
| 4 | miR-766 | 0.62 | 1 / 6 | miR-146b-3p | 73.7+/−112.7 | 3 / 5 |
| 5 | miR-597 | 0.59+/−.2 | 3 / 6 | miR-339-5p | 50.7+/−0.0 | 1 / 5 |
| 6 | miR-211 | 37+/−56.3 | 3 / 5 | |||
| 7 | let-7e | 26.3+/−51.6 | 4 / 5 | |||
| 8 | miR-223* | 26+/−35.5 | 2 / 5 | |||
| 9 | miR-661 | 13.1+/−0.0 | 1 / 5 | |||
| 10 | miR-99a | 13+/−17.7 | 2 / 5 | |||
| 11 | miR-302b | 8.8+/−14.8 | 3 / 5 | |||
| Mouse HDL miRNAs | ||||||
| WT Chow | Apoe −/− HFD | |||||
| Normalized Abundance | ||||||
| miRNA | (+/−SD) | P/A | miRNA | (+/−SD) | P/A | |
| 1 | miR-375 | 48916.24+/−49386.5 | 3 / 3 | miR-383 | 942017.3+/−1332214 | 1 / 4 |
| 2 | miR-223 | 3905.9+/−5671.8 | 3 / 3 | miR-188-5p | 928408.4+/−1856815 | 1 / 4 |
| 3 | miR-17 | 973.8 | 1 / 3 | miR-293 | 222741.1 | 1 / 4 |
| 4 | miR-135a* | 257.42+/−196.9 | 3 / 3 | miR-223 | 12356.26+/−15251.4 | 4 / 4 |
| 5 | miR-709 | 122.5 | 1 / 3 | miR-375 | 312.78+/−2129 | 4 / 4 |
| 6 | miR-466d-3p | 119.4+/−167.34 | 1 / 3 | miR-135a* | 138.1546+/−114.1 | 4 / 4 |
| 7 | miR-141* | 47.16+/−31.6 | 3 / 3 | miR-720 | 136.1308+/−144.1 | 2 / 4 |
| 8 | miR-764-5p | 30.5 | 1 / 3 | miR-466d-3p | 125.6375+/−145.8 | 2 / 4 |
| 9 | miR-720 | 15.6 | 1 / 3 | miR-709 | 58.083+/−2.8 | 2 / 4 |
| 10 | miR-760 | 9.04+/−4.5 | 2 / 3 | miR-764-5p | 31.63+/−5.4 | 2 / 4 |
| Significant Differential Abundance on Apoe −/− HFD HDL | ||||||
| miRNA | Increased Abundance Fold Change | p-value | miRNA | Decreased Abundance Fold Change | p-value | |
| 1 | miR-188-5p | 747272.10 | 0.43 | miR-17 | −107.69 | na |
| 2 | miR-203 | 21.89 | na | miR-375 | −15.71 | 0.05 |
| 3 | miR-685 | 13.78 | 0.09 | miR-99b* | −14.03 | na |
| 4 | miR-24 | 9.13 | 0.30 | miR-801 | −3.23 | na |
| 5 | miR-720 | 8.75 | na | miR-875-5p | −2.55 | 0.19 |
| 6 | miR-877 | 5.77 | 0.48 | miR-30e* | −2.42 | 0.33 |
| 7 | miR-34c | 3.99 | 0.46 | miR-141* | −2.30 | 0.23 |
| 8 | miR-146a | 3.91 | 0.51 | miR-709 | −2.10 | na |
| 9 | miR-378 | 3.33 | 0.05 | miR-30e | −1.95 | 0.03 |
| 10 | miR-223 | 3.16 | 0.41 | miR-135a* | −1.88 | 0.35 |
| OFF | miRNA | ON | P/A | |||
| 1 | miR-383 | 942017.3+/−1332214 | 2 / 4 | |||
| 2 | miR-293 | 222741.1 | 1 / 4 | |||
| 3 | miR-704 | 1.7 | 1 / 4 | |||
| 4 | miR-211 | 1.34+/−0.47 | 2 / 4 | |||
Italicized n.s.; P/A, number of individual profiles specific miRNA is present in top ten ranking / total arrays; SD, standard deviation.