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. 2011 Jan 5;300(4):C755–C770. doi: 10.1152/ajpcell.00360.2010

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Fig. 10. — LC-MS/MS analysis of nonphosphorylated peptides from rat cortical membrane fraction obtained from flow-through fraction of immobilized metal ion affinity chromatography (IMAC) procedure. A: numbers of peptides identified. Individual circles in Venn diagram show the number of phosphopeptides identified using each of three search algorithms (SEQUEST, InsPecT, and OMSSA). A false discovery rate of <2% was used for each of the searches. B: classification of cortical membrane proteins identified by LC-MS/MS. Histogram shows number of proteins (horizontal axis) associated with a particular PANTHER classification term (vertical axis).