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. 2010 Dec 29;300(4):C771–C782. doi: 10.1152/ajpcell.00119.2010

Fig. 5.

Fig. 5.

NHERF2 knockdown abolishes carbachol inhibition of NHE3 activity in Caco-2/bbe cells. NHE3 activity was measured in cells grown on filterslips and infected with Adeno-HA-NHE3. NHERF1 and NHERF2 knock used lentivirus-shRNA and control cells used lentivirus-shRNA GFP. Control cells and NHERF1-KD cells showed 30–40% inhibition of NHE3 activity when treated with 50 μM carbachol for 5 min. The effect of carbachol was abolished in cells with NHERF2 but not NHERF1 knockdown (n = 3). P values are carbachol effect compared with matched untreated controls (paired t-test).