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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Cytokine. 2011 Feb 15;54(2):129–135. doi: 10.1016/j.cyto.2011.01.015

Figure 6. eNOS, p-eNOS, iNOS expression in HMVEC-L.

Figure 6

(A) eNOS expression was determined by quantitative real-time RT-PCR at 21% and 2% O2. There was a significant decrease of eNOS expression at 2% O2. Results were normalized to β-actin expression. Values are mean ± SEM (n=3). **P < 0.01 compared to cells at normoxia. (B) Western blotting analysis confirmed the decreased expression of eNOS and p-eNOS at 2% O2 that persisted with EPO stimulation. iNOS expression was induced at 2% oxygen and even more with 5 U/ml of EPO. (C) Quantification of eNOS, p-eNOS and iNOS in relation to β-actin. (D) Quantification of p-eNOS/eNOS ratio from Western blotting. *P < 0.05 compared to at normoxia. **P < 0.01 compared to cells at normoxia.