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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Nat Cell Biol. 2010 Dec 19;13(1):22–29. doi: 10.1038/ncb2141

Figure 4. Fsd interacts with both Skp1 and Bcd.

Figure 4

(a) A co-IP experiment detecting the interaction between Fsd and Skp1. HEK 293T cells were co-transfected with the indicated combinations of plasmids expressing FLAG-Fsd and HA-Skp1. Anti-FLAG antibody was used to immuno-precipitate FLAG-Fsd in whole cell extracts, followed by Western blotting analyses using anti-HA and anti-FLAG antibodies to detect HA-Skp1 and FLAG-Fsd, respectively.

(b) Same as a, except with the use of a plasmid expressing HA-Bcd (in place of HA-Skp1) to detect the interaction between Bcd and Fsd.