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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Nat Struct Mol Biol. 2011 Jan 23;18(2):222–226. doi: 10.1038/nsmb.1976

Figure 4.

Figure 4

Solvent accessibility of single cysteines in the Z variant of α1AT. The intermediate state of the protein was formed from the denatured state, i.e., upon dilution from high (5M GdmCl) denaturant. Bands of the PEG-modified (-SPEG) and unmodified (-SH) protein in Supplementary Fig. 4a were quantitated to obtain fraction thiol accessibility. Note that refolding of the Z variant yields a state that is not identical to native, and that this accounts for the partial exposure of 332C at the lowest GdmCl concentration.