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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Invest Dermatol. 2010 Dec 30;131(3):613–622. doi: 10.1038/jid.2010.376

Figure 5. UVB stimulates expression of proenkephalin (PENK) mRNA in normal human epidermal keratinocytes and melanocytes.

Figure 5

Keratinocytes: time (at 25 mJ cm−2) (a) and dose (at 1 hour) (b) responses of PENK mRNA expression were measured using Taqman technology. Melanocytes: time (at 100 mJ cm−2) (c) and dose (at 6 hours) (d) responses of PENK mRNA levels were measured using Sybr Green technology. Data are means±SEM (n=3). *P<0.05 versus control. (e) PENK-luc reporter plasmid (lower panel) containing PENK promoter sequence (−772 to +11 bp) and pLINTER cloning vector coding promoterless firefly luciferase (upper panel). (f) UVB stimulates the PENK promoter in AtT-20 cells. After transfection with pPENK-luc, cells were treated with UVB at 25, 50, 75, and 100mJ cm−2. Results represent a fold change in luciferase activity in comparison with control.