Fig. 5.
Overexpression of PDZD11 increases biotin uptake in HuTu-80 cells. HuTu-80 cells were transiently cotransfected with the pcDNA3.1-hSMVT and pFLAG-CMV-2-PDZD11 expression plasmids followed by carrier-mediated [3H]biotin uptake (9 nM; pH 7.4; 3 min) measurement in 24 h posttransfection. Control, nontransfected cells. Values are means ± SE of at least 3 independent experiments. hSMVT alone induced significant (*P < 0.01) induction in biotin uptake compared with control. PDZD11 when coexpressed with hSMVT caused significant (*P < 0.01) increase in biotin uptake compared with hSMVT alone.