Fig. 7.

Effect of PDZD11 siRNA on the cell surface expression level of hSMVT. HuTu-80 cells were transiently transfected with the PDZD11 siRNA or control siRNA (control). Twenty four hours after transfection cells were labeled with EZ-Link Sulfo-NHS-SS-Biotin and lysed, and the equal amount of cell lysates (0.5 mg of total soluble protein) was used for the isolation of the biotinylated proteins with NeutrAvidin agarose. The bound proteins were released with sample loading buffer for SDS-PAGE and analyzed by Western blotting using anti-SMVT antibodies (top). Data were normalized to the total amount of cellular hSMVT (bottom). Image shown represents 3 separate sets of experiments.