Table 1.

Primers used for cloning of SERT promoter constructs

	Primer Sequence
hSERTp1
hSERTp1 (−872/+2)	5′-CGGGGTACCACTCCCGGGCTCAGCTGATCCTCCAC-3′
hSERTp1A (−672/+2)	5′-CGGGGTACCAGCTTTGAACTGTAGCTGGTTAACAA-3′
hSERTp1B (−472/+2)	5′-CGGGGTACCCGGGATGGGGACGATGGGGAGGTGTC-3′
hSERTp1C (−272/+2)	5′-CGGGGTACCGCTCCTCCCTGCGAGCGTGTGTGTGT-3′
Reverse primer	5′-CCCAAGCTTTTGTGCGGAGGGGCGCCGG-3′
hSERTp2
hSERTp2 (1995/+123)	5′-CGGGGTACCATAGGCCGGAGGGAAGTACAGAGG-3′
hSERTp2A (−738/+123)	5′-CGGGGTACCGGTGAGCAGCAGTGCCGTTTAAGG-3′
hSERTp2B (−152/+123)	5′-CGGGGTACCGGACTGCCATGTAGCAAATAGG-3′
Reverse primer	5′-CCCAAGCTTTCCTGCTGGTTAGTAAATGACA-3′

The two alternate promoters of serotonin transporter (SERT) gene, upstream of exon 1a-(referred here as hSERTp1) and upstream of exon 2 and extending upstream of exon 1c (referred here as hSERTp2) were cloned by PCR utilizing gene-specific primers. For both hSERTp1 and hSERTp2, the forward primers were designed to contain an internal site for the KpnI restriction enzyme (underlined) and the reverse primers contained a site for the HindIII enzyme (underlined).