The dependence of logarithmic capacity factor (k´) of NMCs on the concentration of ACN in the mobile phase. Experimental conditions: monolithic column, 40 cm (effective length) /60 cm (total length) × 100µm ID; mobile phase, different concentration of ACN with 5 mM ammonium acetate, pH 6.5; applied voltage, 30 kV; electrokinetic injection, 5 kV for 3s; sample concentration, 100 ng/mL for each. APPI/MS parameters: sheath liquid composition, 50% methanol in water, 5mM ammonium, 2% acetone; sheath liquid flow rate, 5 µL/min; drying gas flow rate, 5L/min; nebulizer pressure, 5 psi; drying gas temperature, 200 °C; vaporizer temperature, 200 °C; capillary voltage, 2500 V; fragmentor voltage, 60 V.