Table 1.

Oligonucleotide-specific primers for PCR of NKCC2 isoforms and NFAT5

Primer	Sequence	PCR Size, bp	Position
F-NKCC2A (m)	5-GGTAACCTCTATCACTGGGT-3		883–902
R-NKCC2A (m)	5-GTCATTGGTTGGATCCACCA-3	237	1120–1101
F-NKCC2B (m)	5-GCCGTGACAGTGACAGCCAT-3		873–892
R-NKCC2B (m)	5-GGATCCACCATCATTGAATCG-3	235	1108–1088
F-NKCC2F (m)	5-GTCGGCTATTTGCACAAACG-3		910–929
R-NKCC2F (m)	5-ATGGAACCGATGATGCGGAT-3	230	1140–1121
F-NFAT5 (m)	5-AACATTGGACAGCCAAAAGG-3		804–823
R-NFAT5 (m)	5-GCAACACCACTGGTTCATTA-3	223	1027–1008

NKCC2, Na⁺-K⁺-2Cl⁻ cotransporter type 2; NFAT, nuclear factor of activated T cells isoform 5; F, forward; R, reverse.