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. Author manuscript; available in PMC: 2011 Apr 12.
Published in final edited form as: Nature. 2010 Jun 16;466(7304):383–387. doi: 10.1038/nature09195

Fig. 3. An intact germline is necessary for longevity and gene expression control by the H3K4me3 regulatory complex.

Fig. 3

a, b, Whole-mount immunofluorescence of young adult worms stained with an ASH-2 antibody (a) or an H3K4me3 antibody (b). Dashed lines marks the germline. Scale bars: 50 μm. c, ash-2 knock-down does not further extend the long lifespan of glp-1(e2141ts) mutant worms that were shifted to the restrictive temperature at the L1 stage. d, set-2 knock-down does not further extend the long lifespan of germline-deficient glp-1(e2141ts) mutant worms that were shifted to the restrictive temperature at the L1 stage. Statistics are presented in Supplementary Table 4. e, Microarray clusters of genes that change significantly upon ash-2 knock-down in wildtype worms, but not in glp-1(e2141ts) mutants. Experimental values are presented in Supplementary Tables 8 and 9. f, Percentage of genes regulated by ASH-2 only in WT worms but not in glp-1(e2141ts) mutants. g, Top gene ontology (GO) terms for genes regulated by ASH-2 in wildtype worms, but not in glp-1(e2141ts) mutants. E.V.: empty vector.