Fig. 4.
Averaged ATP (100 uM)-induced [Ca2+]i increase and NO production in intact UA Endo from luteal, follicular, and pregnant sheep. UA Endo from nonpregnant luteal (n = 7 ewes; top) and follicular (n = 6 ewes; middle) and pregnant (n = 8 ewes; bottom) ewes were dual loaded with fura-2 AM and DAF-2 DA and multiple cells simultaneously imaged as described. Overall responses of all individual cells in each field, regardless of response, were generated to match the equivalent of that previously reported for whole field imaging (by photometry) by Yi et al. (20). Means ± SE of combined animal responses in each group are shown. Data for DAF-2 imaging were also subjected to a curve-fit process and time-dependent changes in eNOS activity were derived (right).