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. 2011 Feb 4;300(4):H1336–H1344. doi: 10.1152/ajpheart.01163.2010

Fig. 2.

Fig. 2.

Inhibition of MEK1/2 reduces PMA, but not ionomycin stimulated superoxide release from NADPH oxidase 5 (Nox5). A: superoxide release from COS-7 cells transfected with hemagglutinin (HA)-Nox5 or a control plasmid (lacZ) in response to PMA (100 nM). RLU, relative light units. B: superoxide release from COS-7 cells expressing Nox5 in the presence and absence of SOD (100 U/ml). C: superoxide from COS-7 cells expressing Nox5 or a control plasmid (lacZ) in response to ionomycin (1 μM). COS-7 cells expressing HA-Nox5 were stimulated with PMA (100 nM; D) or ionomycin (1 μM; E) in the presence and absence of the MEK inhibitor PD-98059 (20 μM). Superoxide in A–E was measured with L-012. F: superoxide release from COS-7 cells transfected with Nox5 was measured using the reduction of cytochrome-c in the presence and absence of SOD (100 U/ml). G: superoxide release was measured from control and PMA-stimulated COS-7 cells expressing Nox5 using cytochrome-c. Cells were preincubated with vehicle (Con) or PD-98059 (PD, 20 μM). H: detection of superoxide using L-012 in COS-7 cells expressing HA-Nox5 in response to PMA (100 nM) or FBS (20%). Results are presented as means ± SE; n = 4–8 experiments. *P < 0.05 vs. control.