Fig. 5.
Overexpression of Hsp22 increases inducible nitric oxide synthase (iNOS) expression. A: increased expression of iNOS in swine myocardium injected with the adenovirus harboring Hsp22 compared with the remote area. Injection with the adenovirus harboring LacZ does not affect iNOS expression. Immunoblotting shows 3 representative examples per group. Bar graph shows means ± SE of n = 6 per group. GAPDH was used as a loading control. *P < 0.05 vs. corresponding remote area. B: correlation between Hsp22 overexpression and iNOS abundance in myocardial samples from 4 different hearts. C: measurement of AAR/LV and IS/AAR in swine myocardium injected with adenovirus harboring LacZ or Hsp22 in the presence or absence of NG-nitro-l-arginine (l-NNA). *P < 0.05 vs. corresponding LacZ; #P < 0.05 vs. Hsp22 without l-NNA.