Fig. 1.
Respiratory activities of brain and spinal cord mitochondria in different metabolic states. A: state 4—resting respiration. B: state 3—oxidative phosphorylation. C: state 3U—uncoupled respiration. D: respiratory control ratios (VState3/VState 4). Incubation conditions: composition of the medium A is described in materials and methods. Chamber volume 550 μl, temperature 25°C. Substrates: glutamate 5 mM + malate 2 mM, pyruvate 2.5 mM + malate 2 mM, succinate 5 mM, ascorbate 5 mM + tetramethyl-phosphonium diacetate (TMPD) 0.3 mM. Additions: mitochondrial protein, 0.3 mg; ADP, 150 μM; cyanide-m-chlorophenylhydrazone (CCCP), 0.4 μM. Results are shown as means ± SE. *P < 0.1; **P < 0.05; ***P < 0.001. NS denotes the absence of statistically significant difference. The data for the spinal cord mitochondria were compared with the corresponding column representing brain mitochondria.