Fig. 2.

Comparison of respiratory activities of the brain and spinal cord mitochondria oxidizing various substrates and their mixtures. A: state 4—resting respiration. B: state 3—oxidative phosphorylation. C: state 3U—uncoupled respiration. Incubation conditions as in Fig. 1. Designations: G + M, glutamate + malate; P + M, pyruvate + malate; G + P + M, glutamate + pyruvate + malate; S, succinate; S + G + P + M, succinate + glutamate + pyruvate + malate. The numbers at the columns with G + M indicate the average respiratory rates in nanomoles O₂ per minute per milligram protein for the brain and spinal cord mitochondria. *P < 0.1; ***P < 0.001. To facilitate comparisons, all data were compared with the first column representing glutamate + malate, taken as 100%.