Skip to main content
. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Cancer Res. 2011 Feb 15;71(7):2664–2674. doi: 10.1158/0008-5472.CAN-10-3055

Figure 5. mAb-mediated depletion of Gr-1+ cells inhibits the glioma development.

Figure 5

Gliomas were induced in C57BL/6 WT mice. The mice with developing gliomas received i.p. injections of anti-Gr-1 mAb (RB6-8C5; 0.25 mg/dose) or control IgG on days 21, 23, 25, and 27 after tumor induction. A, symptom-free survival was monitored. B, total RNA was extracted from the mouse brains to perform quantitative RT-PCR for Cxcl10 mRNA expression levels. C, BILs were isolated to perform flow cytometry for subpopulations of CD11b+Gr-1+ (upper) and CD8+ (lower) cells. Representative flow data (left) and cumulative enumerations from multiple experiments (right) are shown. Numbers in dot plots indicate percentage of gated subpopulations in leukocyte-gated populations; MFI of CD107a on CD8-gated subpopulation is indicated in the bottom panels.