Fig. 2.

Analysis of abundance of soluble RAGE into the media from cultured alveolar epithelial cells stimulated by lipopolysaccharide (LPS) in serum-free conditions (A and B) and in serum-supplemented conditions (C). A: representative results of immunoblot analysis for the RAGE level in the apical side media from consecutive 4 experiments in each study condition. B and C: quantity of soluble RAGE calculated from the RAGE concentration multiplied by the volume of media. LPS increased release of soluble RAGE from cells dose dependently both in serum-free conditions (B) and in serum-supplemented conditions (C) (n = 4 in each preparation, *P < 0.05 vs. control).