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. 2011 Jan 21;300(4):L516–L525. doi: 10.1152/ajplung.00118.2010

Fig. 3.

Fig. 3.

Effect of protease inhibitors on RAGE release from the cultured rat alveolar epithelial cells stimulated by LPS (500 μg/ml). Each figure demonstrates the quantity of RAGE released into the media calculated from the RAGE concentration analyzed by ELISA. When matrix metalloproteinase (MMP) inhibitor-1 (MMPI, inhibitor of MMP-1, -2, -3, -7, -13; A) or CL82198 (a selective MMP-13 inhibitor; B) was added to the medium, release of RAGE under LPS stimulation was dose dependently decreased (n = 4 in each preparation, *P < 0.05 vs. LPS 500 μg/ml without MMP inhibitors). Maximum effect was observed at 20 μM for MMPI and 6 μM for CL82198. C: effect of serine protease inhibitor aprotinin (5–10 trypsin inhibitor units/ml) and cysteine protease inhibitor E-64 (50 μM) on RAGE release induced by LPS treatment. Aprotinin significantly decreased the total amount of RAGE released from alveolar epithelial cells, whereas E-64 did not show significant effect (n = 4 in each preparation, *P < 0.05 vs. LPS).