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RAGE expression on the cell surface: control condition (A), cells treated LPS (500 μg/ml) (B), and cells treated with LPS (500 μg/ml) supplemented with MMP-inhibitor 1 (MMPI, 200 μM) (LPS+MMPI) (C). In each setting, cells were fixed with formalin and stained with anti-RAGE antibody (red) without permeabilization with Triton X, and nuclei were stained with DAPI (blue). Each result was obtained as a representative result from consecutive 4 preparations in each study condition.
