Fig. 5.
PAR2 agonists induce [Ca2+]i changes in airway epithelial cells. Panels from left to right display color maps of [Ca2+]i of 16HBE14o- cells over time in response to application of PAR2 specific agonists. A–D: the peptide agonist SLIGRL-NH2 (80 μM) increases [Ca2+]i in 16HBE14o- cells within 30 s; by 90 s, most cells in the field of view have responded. E–H: the peptidomimetic agonist 2-furoyl-LIGRLO-NH2 (2.5 μM) displays similar [Ca2+]i increases in 16HBE14o- cells. Peptide and peptidomimetic agonist concentrations were chosen to display full Ca2+ response and are representative from an n ≥ 3. Application of filtrate is given as time 0 with all times (s) listed at the top. White lines depict cell borders. A color bar indicating approximate [Ca2+]i is given in the bottom right of the first panel for each experiment.