Fig. 2.

Mycoplasma pneumoniae (Mp) enhances adenovirus-mediated basal TLR2 protein reconstitution in airway epithelial cells of TLR2^−/− mice. TLR2^−/− mice were intranasally inoculated with recombinant mTLR2-Adv or control Adv. Two days later, mice were intranasally treated with saline (Sal) or Mp and killed 24 h later. A: representative photomicrographs of TLR2 immunofluorescence staining (indicated by white arrows) in frozen lung tissue sections from mice with adenoviral treatment and Mp infection (original magnification ×400). B: TLR2 protein was examined in isolated mouse primary tracheal epithelial cells (pooled from the same group) by Western blot. GAPDH protein served as a loading control. Values are means ± SE; n = 4–5/group. Results shown are from one representative experiment of two independent experiments.