Fig. 5.

LTF induction is mainly TLR2 dependent. TLR2^−/− and wild-type (TLR2^+/+) mice were intranasally treated with Sal or Mp and killed 24 h later to examine lung LTF expression. A: whole lung LTF mRNA levels were measured by real-time RT-PCR. Relative levels of mRNA expression were normalized to 18S rRNA levels and calculated via ΔΔCt method. B: densitometric analysis of BAL LTF protein levels examined by Western blot. Values are means ± SE; n = 6/group. NS, not significant (P > 0.05). *P < 0.05.