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. 2011 Jan 14;300(4):L596–L604. doi: 10.1152/ajplung.00029.2010

Fig. 6.

Fig. 6.

Transfection of bPAEC with an adenoviral vector containing the arginase II gene (AdArgII) resulted in more viable cells after a 24-h incubation. A: transfection with AdArgII resulted in a dose-dependent increase in arginase II protein expression in bPAEC. The cells were either not transfected, or transfected with Adβ-gal or AdArgII at either 0.5 or 5 multiplicity of infection (MOI), and 24-h later the protein was harvested for Western blotting for arginase II. AdArgII transfection resulted in a dose-dependent increase in arginase II protein expression, and l-leu had no effect on arginase II protein expression following AdArgII transfection. B: transfection with AdArgII resulted in more viable cells after a 24-h incubation than in Adβ-gal-transfected bPAEC. Inhibiting arginine uptake using pharmacological doses of l-leu prevented the AdArgII-induced increase in viable cell number. Values are means ± SE; n = 4–6 in each group. *AdArgII transfected different from Adβ-gal transfected, P < 0.001. #AdArgII + Leu different from AdArgII, P < 0.001.