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. 2011 Apr 12;9(4):e1000609. doi: 10.1371/journal.pbio.1000609

Figure 5. RA is required for GABAergic differentiation of striatal projection neurons and interneurons migrating to the cortex and olfactory bulb.

Figure 5

Immunofluorescence was performed on E18.5 forebrain coronal sections of wild-type (WT) and Raldh3−/− (KO) embryos. (A–B) Raldh3 immunoreactivity in the subventricular zone of the LGE and septum is lost in the mutant. (C–F) In Raldh3−/− embryos, detection of both Gad67 and GABA is greatly reduced in the LGE, striatum, and cortex (note arrows for Gad67); septum is unaffected. (G–L) Loss of Raldh3 does not affect detection of neuronal marker MAP2, striatal projection marker Foxp1, or astrocytic marker GFAP. (M–N) Raldh3−/− embryos exhibit a reduction of Gad67-positive neurons migrating from the LGE to the olfactory bulb when compared to wild-type. All mutants analyzed generated similar results (n = 3). iz, intermediate zone; LGE, lateral ganglionic eminence; OB, olfactory bulb; P, pallidum; Spt, septum.

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