Table 2.
Optimization of RDT fragment sampling and DNA extraction method
| 1) Selection of RDT fragment | |||
|---|---|---|---|
| Proximal nitrocellulose | Distal nitrocellulose | Filter paper | |
| OptiMal (Qiagen method) | 27.57 | 0 | 39.09 |
| Proximal nitrocellulose | Conjugate pad | Filter paper | |
| SDFK60 (Qiagen method) | 30.55* | 38.62 | 38.62 |
| 2) Selection of DNA extraction method | |||
| Elution method | Qiagen method | ||
| OptiMal (nitrocellulose strip) | 28.59 | 32.64 | |
| SDFK60 (nitrocellulose strip) | 31.53 | 36.12 | |
Cycle threshold (Ct)-values in bold indicate best PCR results (i.e. lowest Ct-values)
* fragment sampling of the nitrocellulose strip together with the conjugate pad gave a Ct-value of 29.28