Table 5.
Detection of mixed infection by PCR on whole blood, PCR on RDT and antigen detection.
| OptiMAL | SDFK60 | ||||
|---|---|---|---|---|---|
| PCR on whole blood | PCR on RDT | Antigen detection | PCR on RDT | Antigen detection | |
| Mixed infection | Ct-value | Ct-value | Test line(s) | Ct-value | Test line(s) |
| P. falciparum +P. malariae | 36.06 + 26.50 | 38.23 + 30.13 | Pan pLDH | 0.00 + 31.17 | negative |
| P. falciparum +P. malariae | 29.68 +33.63 | 32.33 + 39.12 | Pf + pan pLDH | 34.46 + 37.93 | HRP-2 |
| P. falciparum +P. malariae | 25.88 + 37.06 | 27.77 + 39.65 | Pfmx | 29.97 + 0.00 | HRP-2 + pLDH |
| P. falciparum +P. ovale | 36.43 + 25.54 | 0.00 + 28.11 | negative | 40.36 + 30.33 | pLDH |
| P. falciparum +P. ovale | 28.40 + 37.48 | 30.19 + 39.60 | Pfmx | 30.25 + 0.00 | HRP-2 |
Ct-values in bold represent PCR signals obtained for the major Plasmodium species. A negative PCR result (Ct-value = 0.00) indicates a missed minor species.