Table 2.
Effects of different inhibitors on spore viability in the presence of various alleles of MEK1
| % Spore viability (no. tetrads)
|
|||||
|---|---|---|---|---|---|
| 1-Na-PP1
|
1-NM-PP1
|
||||
| Genotype | No I | 0.1 μM | 0.5 μM | 0.1 μM | 0.5 μM |
| MEK1 | 92.7 (72) | 95.5 (72) | 94.0 (96) | 92.1 (48) | 95.8 (96) |
| mek1-as1 | 93.6 (46) | 13.4 (95) | 1.0 (96) | 19.6 (120) | 5.7 (96) |
| mek1-as2 | 76.7 (94) | 14.9 (94) | 2.4 (114) | 5.2 (120) | 1.6 (96) |
| mek1::LEU2 | <1.0 (27) | ||||
| mek1-K199R | 0.9 (58) | ||||
No I, no inhibitor.
The pTS30-based, GST-MEK1-integrating plasmids were transformed into YTS1 ade2. Transformants were patched onto selective medium and then transferred to sporulation medium containing the indicated amount of inhibitor.