Table 3.
Sporulation and spore viability in various mek1 mutants
| Strain/plasmid | Relevant genotype | % spore viability (no. asci) | % sporulationa |
|---|---|---|---|
| YTS4/YEplac181 | mek1 | 2.4 (52) | 83.5 |
| YTS4/pDW15 | 2μ MEK1 | 78.4 (104) | 36.6 |
| YTS4/pDW16 | 2μ mek1-K199R | 5.7 (128) | 78.9 |
| YTS4/pDW17 | 2μ mek1-T327A | 13.9 (182) | 83.3 |
| YTS4/pDW18 | 2μ mek1-T327D | 68.7 (130) | 88.3 |
| YTS4/pDW19 | 2μ mek1-T327S | 54.8 (146) | 90.1 |
| NH423::pTS30::pSB3 | MEK1 RED1 | 94.2 (26) | 84.5 |
| NH423::pRS402::pSB3 | mek1 RED1 | <1.0 (26) | 83.1 |
| NH423::pTS32::pSB3 | mek1-T327A RED1 | 14.4 (26) | 87.0 |
| NH423::pTS33::pSB3 | mek1-T327D RED1 | 51.9 (26) | 92.5 |
| NH423::pTS33::pRS306 | mek1-T327D red1 | 4.0 (25) | 75.0 |
a
Transformants were patched onto selective medium and then transferred to spo plates. Spore viability was monitored by tetrad dissection and sporulation by light microscopy. At least 200 cells were counted for each strain.