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Vac14 and Fig4 do not localize to the E compartment of vps4Δ mutants. The vacuoles and the E compartments of vps4Δ mutants expressing either Vac14-GFP (A) or Fig4-GFP (B) were visualized by FM4-64 staining. The localization of the GFP fusion proteins relative to the FM4-64–positive E compartments (highlighted by white arrow heads) were compared by fluorescence microscopy.
