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. 2004 Jan;15(1):24–36. doi: 10.1091/mbc.E03-05-0297

Figure 8.

Figure 8.

Vac14 interacts with Fig4. Coimmunoprecipitation of Vac14-GFP and Fig4 from P13 and P100 fractions. Lysates from wild-type cells expressing Vac14-GFP were fractionated into P13, S100, and P100 fractions, and Vac14-GFP immunoprecipitated from detergent-solubilized fractions, as described in MATERIALS AND METHODS. Vac14-GFP was resolved by SDS-PAGE and visualized by Western blotting using anti-GFP antibody. Fig4 was detected by Western blotting with anti–Fig4 antisera.