Table 2.
3′-Phosphoinositide levels in wild-type, fig4Δ, vac14Δ, and vac14Δ fig4Δ mutants
| Strain | PtdIns(3)P | PtdIns(3,5)P2 |
|---|---|---|
| Wild-type | 2.60 ± 0.12 | 0.10 ± 0.01 |
| fig4Δ | 1.74 ± 0.02 | 0.07 ± 0.01 |
| vac14Δ | 3.07 ± 0.04 | 0.02 ± 0.01 |
| vac14Δ fig4Δ | 1.70 ± 0.01 | 0.02 ± 0.01 |
PtdIns(3)P and PtdIns(3,5)P2 levels in wild-type, fig4Δ, vac14Δ, and vac14Δ fig4Δ mutants, recorded after HPLC analysis of glycerophosphoinositides derived from the extracted phosphoinositides of [3H]-labeled cells. The values reported are expressed as a percentage of the total [3H]cpm glycerophosphoinositides eluted and are representative of at least three independent experiments.