Table 3.

3-Phosphoinositide levels in wild-type, vac7Δ, vac7Δ fig4Δ, and vac7Δ fig4Δ vac14Δ mutants

Strain	PtdIns(3)P	PtdIns(3,5)P2
Wild-type	2.25 ± 0.12	0.11 ± 0.01
vac7Δ	1.41 ± 0.02	0
vac7Δ fig4Δ	1.81 ± 0.04	0.07 ± 0.01
vac7Δ fig4Δ vac14Δ	1.48 ± 0.04	0

PtdIns(3)P and PtdIns(3,5)P₂ levels in wild-type, vac7Δ, vac7Δ, fig4Δ, and vac7Δ fig4Δ vac14Δ mutants recorded after HPLC analysis of glycerophosphoinositides derived from the extracted phosphoinositides of [³H]-labeled cells. The values reported are expressed as a percentage of the total [³H]cpm glycerophosphoinositides eluted and are representative of at least three independent experiments.