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. 2011 Apr 12;6(4):e18308. doi: 10.1371/journal.pone.0018308

Figure 5. Effect of osajin on the expression of Fas/CD95, FasL/CD95L, GRP78/BiP, CHOP/GADD153 and Bcl-2 family proteins.

Figure 5

TW04 cells were treated with various concentrations of osajin for 24 h. (A) RNA was isolated from cells treated with 5 µM or 7.5 µM osajin. Two µg of RNA was reversely transcribed into cDNA using oligo (dT) primers. RT-PCR analysis was performed using primers specific for Fas, FasL, GRP78, CHOP, Bax and Bcl-2 genes and also the internal control gene, β-actin. (B) Cell lysates were prepared for SDS-PAGE followed by Western blot for Fas, FasL, GRP78, CHOP, Bax and Bcl-2, with β-actin as a loading control. (C) to (E) Results are presented as the relative densities of protein bands normalized to β-actin. The data shown are the means ± SE of three individual experiments (*P<0.05 versus 0 µM control).