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. 2011 Apr 12;6(4):e18308. doi: 10.1371/journal.pone.0018308

Figure 7. Co-immunoprecipitation of Bax and Bcl-2 in osajin-treated NPC cells.

Figure 7

Immunoprecipitation assays were preformed as described in Materials and Methods. TW04 cell lysates served as a positive control; while immunoprecipitation wash buffer served as a negative control. Cell lysates of untreated and osajin-treated TW04 cells were immunoprecipitated by primary anti-Bax or anti-Bcl-2 antibodies. Western blots were preformed to demonstrate whether osajin treatment affected the heterodimerization of Bax and Bcl-2.